Glowy Glowy Dots!

The piece of DNA provided was called plasmid. The outer part has amp. resistance. There's a hunk of DNA that allows this piece of antibacterial to live in this antibiotic. Green Florescent Protein (which has been known to glow-can make mice glow!) Ara promoter is put next to the green gene. If the DNA is grown in the ara, then it turns on the green gene. For bacteria to turn green, it must be plus (plus vile and negative vile)

Some labelled LB (everything grows on this one!), some LB/amp (tend to have colonies, don't have the resistance gene), and LB/amp/ara (individual colonies, and should glow!)

Here's our steps!
Slap that bacteria on ice! (gently, though!)
include plasmid
heat a little
throw on ice for a heat shock
examine!

So the pictures aren't available, but I will describe them to you! The tube with the living DNA in it grew tons of tiny dots that seemed to glow when under flashlight. But the dead DNA tube looked like toothpaste smudges and had no dots or glow.

It's even been proven that cats with this gene tend to glow under certain light!




So here's a fun thought to leave you with...If you had this gene...

WOULD YOU GLOW...??