Friday, December 14, 2012

Photosynthesis Dry Lab



Photosynthesis Dry Lab

In this “dry lab” you will be working backwards from what you would normally do in a lab situation. In this case, you will be given a set of observations that were made in a lab and you will be asked to reconstruct the procedure that could have generated this data. You will also be given a set of facts that you will use to explain this set of observations in the analysis and conclusions section of your lab write-up. As you create the procedure for this lab, please remember all the rules that you have been learning about good experimental design.

Your lab report begins below. Fill in the missing sections of the lab report using the observations and facts given. You may assume that you have access to as many test tubes, snails, Elodea plants, light sources, dark places, and as much pond water and BTB as you need.

Make your own copy of this Google Doc and edit the lab report that begins here:


My (only) Most Awesomest Photosynthesis Lab Report
by Cynthia Justice

Purpose: Is photosynthesis possible in animals? With different chemicals? Is light necessary?

Background Facts:
  • Carbon dioxide in water produces carbonic acid.
  • Bromothymol Blue (BTB) is a blue-green liquid which changes to a yellow color in acid and back to blue-green when returned to a neutral pH.
  • Carbon dioxide plus water yields sugar and oxygen when chlorophyll and sunlight are present.
  • Animals respire.
  • Green plants photosynthesize in the light and respire all the time.
  • Sugar plus oxygen yields carbon dioxide plus water and energy.


Hypothesis:
If I mix bromothymol with other ingredients in different conditions, the mixture will change colors.
Materials:
4 beakers
bromothymol blue
2 aquarium fish
2 elodea
water
Procedure:
1.  Fill a beaker 3/4 full of water and mix with 40 drops of  bromothymol blue (BTB).  Leave in light for approx. 3 hrs.  Then leave in dark for 3 hours.  Observe!
2.  Fill a beaker 3/4 full of water and mix with 40 drops of BTB.  Put an aquarium fish in with the mixture.  Put in light for 3 hrs.  Put in dark for 3 hrs.  Make observations.
3.  Fill a beaker 3/4 full of water and mix with 40 drops of BTB.  Add an elodea to the mixture.  Leave in light for 3 hrs.  Then in dark for 3 hrs.  Make observations.
4.  Fill a beaker 3/4 full of water and mix with 40 drops of BTB.  Put both an aquarium fish and an elodea in the mixture.  Put in light for 3 hrs.  Put in dark for 3 hrs. Make those final observations!



Observations:

  1. Water plus bromothymol blue is blue-green.
  2. Water plus bromothymol blue plus an aquarium snail turns yellow.
  3. Water plus bromothymol blue plus Elodea (an aquarium plant) is blue-green in light.
  4. Water plus bromothymol blue plus a snail plus Elodea is blue-green in light and yellow when left in the dark for three hours.

Analysis and Conclusions:

  1. Water plus bromothymol blue is blue-green because...
the water is a neutral pH
  1. Water plus bromothymol blue plus an aquarium snail turns yellow because...
the animal respires and produces carbon acid
  1. Water plus bromothymol blue plus Elodea (an aquarium plant) is blue-green in light because...
the pH levels of plants stay neutral in the light and photosynthesis process, so the water stays the same color
  1. Water plus bromothymol blue plus a snail plus Elodea is blue-green in light and yellow when left in the dark for three hours...
the snail produces carbon dioxide, but there is no light, so the elodea can't go through photosynthesis and the carbon dioxide makes the water acidic, which creates the chemicals reaction and changes the water color in the dark.

Phenylketonuria Web Quest


PHENYLKETONURIA: A METABOLIC DISORDER

Metabolic disorders are genetic diseases that affect the body's ability to perform its normal chemical reactions. Many metabolic disorders result from enzyme defects. Recall that a metabolic pathway is a stepwise sequence of enzyme-mediated reactions. If one enzyme in a metabolic pathway is defective, that enzyme's substrate may accumulate and the pathway may not be completed. This may result in a buildup of harmful substances or a shortage of required molecules.

Activity

In this exercise, you will use the Web links below to gather information about the metabolic disorder phenylketonuria (PKU). Use what you learn to answer the questions at the bottom of the page.


Your Genes, Your Health: Phenylketonuria


NSPKU Home Page

Texas Department of Health Genetic Disorders

Phenylketonuria - The Genetics


Questions

1. What enzyme is most commonly defective in people with phenylketonuria?
An enzyme called Phenylalanine Hydroxylase 
2. What reaction does this enzyme catalyze? (What is the substrate and what product is produced?)
PAH. Without this, a person could develop high levels of phenylalanine in the brain, which could poison neurons and cause mental retardation or epilepsy.
3. Describe the symptoms of phenylketonuria.
Smaller than normal head, mental retardation, epilepsy, musty odor, and lighter skin and hair4. What causes the symptoms of PKU, the lack of a substance or the buildup of one?
Phenylalanine reacts badly with PKU because the hindered enzyme is one of a metabolic nature.5. How common is phenylketonuria? How is it treated?
It's not very common at all. It's treated by a low-protein diet that is carried out throughout the patient's life.