Showing posts with label pictures. Show all posts
Showing posts with label pictures. Show all posts

Friday, December 14, 2012

Photosynthesis Dry Lab



Photosynthesis Dry Lab

In this “dry lab” you will be working backwards from what you would normally do in a lab situation. In this case, you will be given a set of observations that were made in a lab and you will be asked to reconstruct the procedure that could have generated this data. You will also be given a set of facts that you will use to explain this set of observations in the analysis and conclusions section of your lab write-up. As you create the procedure for this lab, please remember all the rules that you have been learning about good experimental design.

Your lab report begins below. Fill in the missing sections of the lab report using the observations and facts given. You may assume that you have access to as many test tubes, snails, Elodea plants, light sources, dark places, and as much pond water and BTB as you need.

Make your own copy of this Google Doc and edit the lab report that begins here:


My (only) Most Awesomest Photosynthesis Lab Report
by Cynthia Justice

Purpose: Is photosynthesis possible in animals? With different chemicals? Is light necessary?

Background Facts:
  • Carbon dioxide in water produces carbonic acid.
  • Bromothymol Blue (BTB) is a blue-green liquid which changes to a yellow color in acid and back to blue-green when returned to a neutral pH.
  • Carbon dioxide plus water yields sugar and oxygen when chlorophyll and sunlight are present.
  • Animals respire.
  • Green plants photosynthesize in the light and respire all the time.
  • Sugar plus oxygen yields carbon dioxide plus water and energy.


Hypothesis:
If I mix bromothymol with other ingredients in different conditions, the mixture will change colors.
Materials:
4 beakers
bromothymol blue
2 aquarium fish
2 elodea
water
Procedure:
1.  Fill a beaker 3/4 full of water and mix with 40 drops of  bromothymol blue (BTB).  Leave in light for approx. 3 hrs.  Then leave in dark for 3 hours.  Observe!
2.  Fill a beaker 3/4 full of water and mix with 40 drops of BTB.  Put an aquarium fish in with the mixture.  Put in light for 3 hrs.  Put in dark for 3 hrs.  Make observations.
3.  Fill a beaker 3/4 full of water and mix with 40 drops of BTB.  Add an elodea to the mixture.  Leave in light for 3 hrs.  Then in dark for 3 hrs.  Make observations.
4.  Fill a beaker 3/4 full of water and mix with 40 drops of BTB.  Put both an aquarium fish and an elodea in the mixture.  Put in light for 3 hrs.  Put in dark for 3 hrs. Make those final observations!



Observations:

  1. Water plus bromothymol blue is blue-green.
  2. Water plus bromothymol blue plus an aquarium snail turns yellow.
  3. Water plus bromothymol blue plus Elodea (an aquarium plant) is blue-green in light.
  4. Water plus bromothymol blue plus a snail plus Elodea is blue-green in light and yellow when left in the dark for three hours.

Analysis and Conclusions:

  1. Water plus bromothymol blue is blue-green because...
the water is a neutral pH
  1. Water plus bromothymol blue plus an aquarium snail turns yellow because...
the animal respires and produces carbon acid
  1. Water plus bromothymol blue plus Elodea (an aquarium plant) is blue-green in light because...
the pH levels of plants stay neutral in the light and photosynthesis process, so the water stays the same color
  1. Water plus bromothymol blue plus a snail plus Elodea is blue-green in light and yellow when left in the dark for three hours...
the snail produces carbon dioxide, but there is no light, so the elodea can't go through photosynthesis and the carbon dioxide makes the water acidic, which creates the chemicals reaction and changes the water color in the dark.

Thursday, November 29, 2012

Metabolism Intro Notes

Forms of Energy
    Law of thermodynamics
    Metabolic reactions
ATP
Metabolic Pathways
-energy of activation-enzymes
-photosynthesis
-cellular respiration


2 Types of energy:
Kinetic
-energy of motion
-mechanical

Potential
-Stored energy
-chemical


Laws of Thermodynamics
First law:
-law of conservation of energy
-energy cannot be created or destroyed
-energy CAN be changed from one form to another
Second Law:
-Law of Entropy
-when energy is changed from one form to another, there is a loss of usable energy
-waste energy goes to increase disorder

Metabolic Reactions and Energy Transformations
Metabolism:
-sum of cellular chemical reactions in cell
-reactants participate in reaction
-products form as result of reaction
Free energy s the amount of energy available to perform work
-Exergonic Reactions-Products have less free energy than reactants
-Endergonic reactions-Products have more free energy than reactants

ATP and Coupled Reactions
Adensonine Triphosphate (ATP)
-High energy compound used to drive metabolic reactions
-constantly being generated from adenosine diphosphate (ADP)
Composed of:
-adenine and ribose (together=adensosine)
-three phosphate groups
Coupled reactions
-energy released by an exergonic reaction captured in ATP
-that ATP used to drive an endergonic reaction

ATP= ADP + P + Energy



Work-Related Functions of ATP
Primarily to perform cellular work
-Chemical work-Energy needed to synthesize macromolecules
-Transport work-Energy needed to pump substances across plasma membrane
-Mechanical work-Energy needed to contract

Metabolic Pathways
-Products of an earlier reaction become reactants of a later reaction
-Such linked reactions form a metabolic pathway
   -Begins with a particular reactant
   -Proceeds through several intermediates
   -Terminates with a particular end product

Enzymes
-Protein molecules that function as catalysts
-the reactants of an enzymatically accelerated reaction are called substrates
-each enzyme accelerates a specific reaction
-each reaction in a metabolic pathway requires a unique and specific enzyme
-end product will not appear unless ALL enzymes present and functional

Enzymes: Energy of Activation
Reactants often "reluctant" to participate in reaction
-energy must be added to at least one reactant to initiate the reaction
-energy of activation
Enzyme Operation:
-Enzymes operate by lowering the energy of activation
-Accomplished by bringing the substances


Irreversible Inhibition
Materials that irreversibly inhibit an enzyme are known as poisons
Cyanides inhibit enzymes resulting in all ATP production
Penicillin inhibits an enzyme unique to certain bacteria
Heavy metals irreversible bind with many enzymes
Nerve gas irreversibly inhibits enzymes required by nervous system



Thursday, November 15, 2012

Microscopy

Your supplies:
2 Artifacts
1 Test Tube

Now let's do this!


The main objective in this project is to test the ability to use a microscope. Objectives will be tested.

Start off on lowest magnification possible, find the desired area, and magnify!

There is a part just below the microscope call the condenser. It has an iris under microscope. If no light is coming through, the iris must be twisted. So of course, this is a sign that there's a little bit of microscope anatomy we need to touch up on first!

There!
First, we tested hair! The outcome was very interesting. It looked like the following:

JUST KIDDING!

It REALLY looked like...


Next, we checked out some infected pork! That one was very interesting to see up close! It looked somewhat like the following...

Notice the three chunked spots. That, we concluded, is the infection. Pretty nasty, but neat, right??

Microscopes are awesome! Just with one little peek through, one's world can be completely magnified! Very nifty tool that everyone should have to experience in playing with!